Plasmid

Part:BBa_K4259003:Design

Designed by: Jennifer Adami   Group: iGEM22_Leiden   (2022-09-27)


CopA


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 2481
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 597
    Illegal NgoMIV site found at 2039
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1081
    Illegal SapI.rc site found at 324


Design Notes

Codon optimization was performed for the expression of the gene in E. coli.


Source

The sequence of CopA was obtained from UniProt

References